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News, Calendars, and Events » Calendars » Master Calendar » Biochemistry and Molecular & Cellular Biology, Department of
Seminar Series: Dr. Paul Vouros
Schedule information
Event Seminar Series: Dr. Paul Vouros
When Tuesday, January 29, 2013 from 12:00pm to 1:00pm
Where Basic Science Building 341 (Library)
Event details
Details 'Analysis of DNA and Other Biomarkers by Mass Spectrometry: Transitioning from LC-MS to DMS-MS'

Dr. Paul Vouros
Department of Chemistry and Chemical Biology
Northeastern University, Boston, MA

Abstract: HPLC-MS is presently recognized as the premier method of choice for addressing a broad range of bioanalytical problems. Our laboratory has been active for many years in the development of HPLC-MS based related methodologies. One such example is the analysis of biomarkers derived from cellular exposure to toxic agents. These agents or their electrophilic metabolites can bind covalently to DNA to form DNA adducts. It is generally accepted that DNA adducts represent an early, critical, and detectable step in carcinogenesis, and may serve as useful biomarkers for the risk assessment of exposure to harmful environmental carcinogens.

In order to meet detection and quantitation requirements for DNA adducts and other drugs or metabolites, our laboratory has developed analytical protocols utilizing nanoelectrospray ionization in combination with nanoflow HPLC. While effective, a major limitation of our NanoLC – Nano-ESI approach is the low sample throughput. This problem is further exacerbated by the fact that the analysis is typically preceded by several time-consuming sample cleanup steps.

In view of the above, we have always been interested in exploring new analytical technologies. Differential Mobility Spectrometry (DMS) has drawn our attention in this regard if used in combination with tandem mass spectrometry and has been used as a rapid gas phase ion separation/ filtration technique. Although DMS has been primarily used for separations, it has been demonstrated that its unique capability allows pre-filtering of ion species prior to mass analysis resulting in reduced noise and enhanced sensitivity. This presentation will discuss the use of a miniature planar DMS interfaced to MS in forensic applications and in the analysis of biomarkers associated with DNA damage from environmental carcinogens. As compared to traditional LC-MS based techniques, it is shown that DMS-MS is capable of separating mixtures of moderate complexity and quantifying targeted analytes in less than one minute of analysis time.
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Contact Juanita Chipani Biochemistry and Molecular & Cellular Biology office, x71512
Sponsors Department of Biochemistry and Molecular & Cellular Biology
Calendar Biochemistry and Molecular & Cellular Biology, Department of
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